Fig. 8

2-BP delays tumor growth in Panc 02 tumor model. A–D Individual C57BL/6 mice were subcutaneously injected with 5 × 10⁶ Panc 02 cells and received 2-BP treatment or Vehicle (CTRL). Schematic representation of treatment plan (A). Representative images of the tumors taken 28 days after inoculation, individuals’ representative tumor of each group (B), tumor weight (C), and tumor growth curves (D) of Vehicle (CTRL) or 2-BP treated Panc 02 tumors (n = 8 mice per group). E Western blotting analysis showing the decreased PD-L1 protein expression in Panc 02 tumor tissues of mice treated with 2-BP (50 mg/kg) at the indicated time. F, G H&E and IHC staining (F) of PD-L1, CD8α, and GZMB in Panc 02 tumor tissues of mice treated by 2-BP (50 mg/kg) and quantification (G). H, I Flow cytometry analysis of the number of tumor-infiltrating CD8⁺ T cells (CD45⁺ CD8⁺) (H) in CTRL and 2-BP treated Panc 02 tumors and quantification (I). J Concentrations of CCL4, CCL5, CXCL9, and CXCL10 in CTRL and 2-BP treated Panc 02 tumor tissues were measured by ELISA. Results shown are presented as mean ± SD. (ns, not significant, **p < 0.01, and ***p < 0.001)