Fig. 7

2-BP suppresses pancreatic-tumor cell viability and tumor sphere-forming activities, as well as increases apoptosis in vitro. A, B The effect of 2-BP on the apoptosis of Panc 02 (A) and PANC-1 (B) cells were detected by flow cytometry. C, D The Panc 02 (C) and PANC-1 (D) cells were pre-treated with 2-BP for 24 h, then the cell viability was analyzed using the CCK8 cell proliferation assay kit. E, F The effect of 2-BP treatment on the migration of Panc 02 (E) and PANC-1 (F) cells were examined by scratch test. G, H Representative images showing Panc 02 (G) and PANC-1 (H) 3D tumor culture model maintained in tumorsphere conditions for 7 days with indicated 2-BP treatment. I, J Differences in the expression of stem cell markers (ALDH1, Nanog, OCT4, SOX2) and mTOR signaling in Panc 02 (I) and PANC-1 (J) tumor spheres treated with 2-BP was analyzed by Western blotting assay. K The effect of 2-BP on the apoptosis of HPDE6-C7 cells was detected by flow cytometry. L The effect of 2-BP treatment on the migration of HPDE6-C7 cells was examined by scratch test. M The effect of 2-BP treatment on the HPDE6-C7 cell proliferation was examined by CCK8 cell proliferation assay kit. N Cell cycle of HPDE6-C7 cells with or without 2-BP treatment was analyzed by flow cytometry. The percent of G0/G1 phase, S phase, and G2/M phase were determined. Results shown are presented as mean ± SD. (ns, not significant, *p < 0.05, **p < 0.01, and ***p < 0.001)