Fig. 5

Notch1 directly regulated STC1 expression to establish a paracrine amplifying STC1-Notch1 feedforward signal. A Notch1 was knocked out in HCC cells, and western blotting was used to measure the expression levels of Notch1, NICD, and STC1. B, C Western blot (B) and qRT‒PCR (C) analyses were used to quantify the expression of Notch1 and STC1 in MHCC-97H and SNU-398 cells. D The CSL binding motif. E Mutation of possible binding sites of CSL and STC1 promoter sequences. F, G Luciferase reporter assays were performed to measure luciferase activities in MHCC-97H (F) and SNU-398 (G) cells. Renilla luciferase activity was used as the internal reference. For the statistical analysis, *P < 0.05, **P < 0.01, and ***P < 0.001, t test