Fig. 1
From: Pharmacologic inhibition of IL11/STAT3 signaling increases MHC-I expression and T cell infiltration
IL11 knockout increases CD8+ T cell infiltration and reduces intestinal and colon tumorigenesis. A Schematics of AOM/DSS administration and analysis time points (n = 8 mice/group). C57BL/6 or Il11−/− mice (n = 8 per group) were intraperitoneally (i.p.) injected with 10 mg/kg body weight (BW) of AOM, and kept on regular diet and water for 7 days. After 7 days, mice received water with 2.5% DSS for 7 days, and resume to regular diet and water. Afterwards, mice received a second 7-day administration of DSS on day 28, and on day 49 a third. All mice were sacrificed and analyzed on day 86. B Colonoscopy images of AOM/DSS-induced CAC in WT and Il11−/− mice on day 86. C Representative images of WT and Il11−/− colon and rectum autopsy on day 86 (stained with methylene blue). D Tumor count of WT and Il11−/− colon and rectum (n = 8 mice/group). *P < 0.05, t test. E Tumor diameter (mm) of WT and Il11−/− colon and rectum (n = 8 mice/group). ns, no significance; *P < 0.05, t test. F Immunofluorescence and cell counts of CD3 (red), CD8 (green) and DAPI for nuclei (blue) in WT and Il11−/− colon tumors (n = 6 mice/group). Scale bar, 50 μm. *P < 0.05, **P < 0.01, t test. G Representative images of 14-week old Apcmin/+ and Il11−/−/Apcmin/+ intestine autopsy (stained with methylene blue). H Tumor count of Apcmin/+ and Il11−/−/Apcmin/+ intestine (n = 8 mice/group). **P < 0.01, t test. I Representative images of 14-week old Apcmin/+ and Il11−/−/Apcmin/+ colon and rectum autopsy (stained with methylene blue). J Tumor count of Apcmin/+ and Il11−/−/Apcmin/+ colon and rectum (n = 8 mice/group). *P < 0.05, t test