Fig. 3

Mutation Profiles across Different HER2 Statuses. A Oncoplot of genomic alterations stratified by different subtypes from our NGS cohort. B The differentially mutated genes between different subtypes shown by forest plot in our NGS database. Only differentially mutated genes with statistical significance are shown, and the x-axis reports the odds ratio with 95% confidence intervals (CIs). < 1 represents more mutants in the left subtype, while > 1 represents more mutants in the right subtype. C Spider plot comparing the HR+HER2-low (left) or HR−HER2-low (right) group and the other subtypes. On the y-axis of the spider plot, the number of genes with a significantly different number of mutations (Fisher's exact test) between the reference group and each control group was reported. For instance, when considering the whole HR+HER2-low cohort, 5 differentially mutated genes with respect to the HR+HER2-positive cohort and 3 for the HR+HER2-zero cohort. When the whole HR−HER2-low cohort was compared with HR−HER2-positive tumors, we detected 5 differentially mutated genes with respect to the HR−HER2-positive cohort and 1 for the HR−HER2-zero cohort. D. The mutation rates of PIK3CA, TP53, PTEN, RB1, BRCA1 and ESR1 according to HR and HER2 status in our NGS database. The P value was calculated by the Chi-square test or Fisher's exact test based on the number of cases per group featuring the presence or absence of the gene mutation. E The number of patients with germline mutations in each subtype and in each individual gene. * 0.05 > p value ≥ 0.01, ** 0.01 > p value ≥ 0.001, *** 0.001 > p value ≥ 0.0001, **** p value < 0.0001