Fig. 9

Inhibition of ROS production sensitizes HCC cells to hyperthermia in vivo. NAC (100 mg/kg/day) was administered via tail vein injection starting 2 h after iRFA for 5 days. A and B A significantly higher portion of TUNEL positive cells was observed in iRFA + NAC Group 1 day after iRFA. The tumors were collected on Day 21. Tumor growth was monitored at the indicated times. The C tumor weights and D tumor volume growth curves indicated that NAC sensitized HepG2 cells to hyperthermia. E Image showing the tumor burdens in mice. F Schematic diagram of the ROS burst that is involved in cell survival after sublethal heat stress. Values are the mean ± SE.**p < 0.01;***p < 0.001; ns no significance