Fig. 8

Nrf2 was elevated in response to oxidative stress after sublethal heat stress and mediated ROS induced mitophagy. A Western blot of Keap1 and Nrf2 expression in whole cell lysates. B Changes in the protein expression of Nrf2 in nucleus. C Representative image of Nrf2 localization in cells in different groups.DEffect of NAC(5 mM) on Nrf2 E Effect of adding NAC(5 mM) 12 h after sublethal heat stress on Nrf2. F Inhibition effect of NAC on PINK1 after sublethal heat stress was reversed by bardoxolone. G Apoptosis induced by NAC after sublethal heat stress was reversed by bardoxolone at 24 h after sublethal heat stress