Fig. 6

Mitophagy is induced by sublethal heat stress and is involved in survival. A WB of GAPDH, P62, LC3B, and TOMM20. B Representative confocal images of cells coloaded with MitoTracker™ Red and LysoTracker™ Green at 24 h after sublethal heat stress. Colocalization is shown in yellow and indicated by arrows. C Flow cytometry analysis of apoptosis of heated or unheated HCC cells after inducing or inhibiting mitophagy at 24 h after sublethal heat stress. Values are the mean ± SE.**p < 0.01; ***p < 0.001; ns no significance