Fig. 5

Inhibition of ROS production results in accumulation of damaged mitochondria and severe mitochondrial dysfunction after sublethal heat stress. A The mitochondrial DNA copies of each group. B Flow cytometry of mitochondrial status. Gates represent cells with damaged mitochondria. C The statistical results of B in three independent experiments. D Representative images of mitochondrial morphology. E Mitochondrial as mass assessed by staining with MitoTracker™ Green using a flow cytometric. F ATP contents of each group. G Results of the Mito stress test. Values are the mean ± SE.*p < 0.05;**p < 0.01; ***p < 0.001; ns no significance