Fig. 7

Uric acid treatment increases FASN/SREBP1 expression and FFA accumulation in HepG2 and can be rescued via SREBP1 knock down. A Schematic diagram of the cell culture, HepG2 were transfected with SREBP1 siRNA or control siRNA for 24 h followed by 1 mM UA treatment for another 48 h. The SERBP1 and FASN mRNA and protein levels, and fatty acids were measured and analyzed then. B Column plot of SERBP1 and FASN mRNA expression. N = 6 per each group. The average values of Si-Ctrl were standardized to 1, *p < 0.05 as compared to control group; For statistical analysis one-way ANOVA followed by Sidak’s multiple comparison test was applied, ^#p < 0.05 as compared to UA group. C Western blot of SERBP1 and FASN protein expression, n = 3 per group. SREBP1 signaling can be detected as an ~ 68 splicing and 120 kDa full-length protein bands. D Heatmap showed uric acid positively correlated to several lipids in HepG2 cell and can be rescued via SREBP1 knock down. The color in each cell represents value of correlation calculated by Spearman’s rank correlation coefficient. *p < 0.05 as compared to control group; ^#p < 0.05 as compared to UA group. E Venn diagram displays the distribution of shared FFA alteration after uric acid treatment in cell, zebrafish and human dataset. FASN, fatty acid synthase; SREBP1, sterol regulatory element binding protein 1