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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Atf4 regulates angiogenic differences between alveolar bone and long bone macrophages by regulating M1 polarization, based on single-cell RNA sequencing, RNA-seq and ATAC-seq analysis

Fig. 4

Vegfa with high expression in ABM macrophages. A Expression of the selected four ligands secreted by ABM macrophages and LBM macrophages. B Expression of the four ligands in ABM macrophages and LBM macrophages was analyzed by PCR assay. C ELISA for Vegfa in ABM and LBM conditioned medium. D Comparison of Vegfa expression in ABM macrophages and LBM macrophages by WB. E Quantification of the expression of Vegfa in ABM macrophages and LBM macrophages. F Expression of Vegfb, Vegfc, Vegfd, Fgf2 in ABM macrophages and LBM macrophages was analyzed by PCR assay. *Corrected P-value < 0.05. **Corrected P-value < 0.01. ABM alveolar bone marrow, LBM long bone marrow, Vegfa vascular endothelial growth factor A, Vegfb vascular endothelial growth factor B, Vegfc vascular endothelial growth factor C, Vegfd vascular endothelial growth factor D, Fgf2 fibroblast growth factor 2, PCR polymerase chain reaction

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