Fig. 4

Transcriptomic clusters in the PTMETA and TCGA cohorts. Heatmaps depicting the expression of the top 20 AUROC-ranked marker genes in each subgroup of the (a) PTMETA cohort and (b) PTMC-TCGA cohort. The main enriched KEGG terms of all marker genes in each subgroup are listed on the right; (c) Subclass mapping of subgroups found in the PTMETA and PTMC-TCGA cohort. Significant correspondence between subgroups is highlighted in blue with Bonferroni adjusted p-values; (d) Comparison of the tumor immune microenvironment across different subgroups in the PTMETA cohort using two methods: ESTIMATE immune scores, GSVA using Bindea et al.’s combined immune gene set; (e) Comparison of the immune cell fraction distinguished by the different subgroups; (f) Comparison of IFN-γ scores, PDL1, PDCD1, and CTLA4 expression. p-values from the Wilcoxon rank-sum tests. PTMC-Pro: PTMC-proliferation; PTMC-Inf: PTMC-inflammatory; PTMC-Met: PTMC-Metabolism