Fig. 6
From: EGR1 induces EMT in pancreatic cancer via a P300/SNAI2 pathway
EGR1 interacted with p300/CBP to activate SNAI2 transcription. A The relative mRNA expression of SNAI2 was downregulated after treatment with GNE-272 in BxPC-3 and PANC1. B The expression of p300 and SNAI2 protein were downregulated after treatment by GNE-272 in BxPC-3 and PANC1. C The relative mRNA expression of SNAI2 was upregulated after EGR1 overexpression and reduced to the baseline after further treatment with GNE-272. D The expression of SNAI2 protein were upregulated after EGR1 overexpression and significantly reduced after further treatment with GNE-272. E The blot image of Co-IP assay showed that EGR1 directly interacted with p300 and CBP. F The blot image of Co-IP assay showed that p300 directly interacted with EGR1. G The results of ChIP-qPCR showed that the combination of EGR1 and SNAI2 promoter regions was downregulated after treatment with GNE-272. *: p < 0.05; ***: p < 0.001