Fig. 4

Different gut microbiota distribution and metabolites may contribute to individualized TMZ efficacy. A The α-diversity indexes of Chao1, Shannon and Simpson of TMZ S and NS mice gut microbiota. B The β-diversity indexes evaluated by weighted-wilcox of gut microbiota between TMZ S and NS individuals. C Significantly changed bacterial genera between S and NS mice. D OPLS-DA score plot based on LC–MS ( +) (R²X = 0.236, R²Y = 0.794, Q² = -0.522) and LC–MS (-) (R²X = 0.475, R²Y = 0.808, Q² = 0.569) data. E Heatmap of Spearman correlation coefficient between pharmacodynamics indices after TMZ treatment and abundance of changed bacterial genera/metabolites. The intensity of the colors represents the degree of association between the level of pharmacodynamics indices and abundance of changed bacterial genera/metabolites determined by Spearman’s correlations. The p-values < 0.05 were considered statistically significant, *p < 0.05, **p < 0.01