Fig. 4

TREM-1 activation induces necroptosis through mitochondrial fission in macrophages. A The cell survival rate, n = 5. B–D Protein levels of RIPK3, MLKL, and their phosphorylation, n = 3. E Immunofluorescence staining for MLKL (green); scale bars = 5 μm. F The concentration of TNF‐α in the supernatant, n = 3. G, H The level of IL-1β p17 in macrophages supernatant, n = 3 biological replicates