Fig. 3

CircDNAJC11 promotes the proliferation of BC cells in vitro. A–D Relative expression of circDNAJC11 and DNAJC11 in BC cells transfected with indicated vectors and siRNAs was determined by qRT-PCR. E The proliferative activity of the cells was measured using CCK-8 assay after overexpression or knockdown of circDNAJC11 expression in MCF-7 and SK-BR-3 cells. F and G The effect of circDNAJC11 on the proliferative capacity of MCF-7 and SK-BR-3 cells was analyzed using a colony formation assay. The left panel F was the representative figure, and the right panel G showed the statistical analysis. H and I EdU experiment was implemented to evaluate the effect of overexpression or knockdown of circDNAJC11 on the malignant proliferation of BC cells (upper panel) and statistical analysis (lower panel). For A–D, β-actin was utilized a loading control. Data were presented as mean ± SD and representative of three independent experiments in A–E, G, and I. For the above experiments, the Student's t-test was used for comparison between two groups, and the ANOVA test was used for three groups. * P < 0.05, ** P < 0.01, ***P < 0.001, ns, no significance