Fig. 1

The identification of circDNAJC11. A, B Heatmap and scatter plot of the differentially expressed circRNAs in 4 paired BC tissues and adjacent non-tumor tissues using the RNA Microarray analysis. C Schematic representation of the DNAJC11 in the generation of the circDNAJC11. Sanger sequencing for the verification of the reverse splicing site of circDNAJC11. D CircDNAJC11 and β-actin were amplified from the cDNA or gDNA of MCF-7 cells using their divergent primers and convergent primers respectively. E The relative expression levels of circDNAJC11 and DNAJC11 mRNA were determined in MCF-7 cells with or without Act D treatment by qRT-PCR. F The relative expression levels of circDNAJC11 and DNAJC11 mRNA were evaluated in the Rnase R-treated RNA samples by qRT-PCR. G RNA nucleocytoplasmic separation combined with qRT-PCR was utilized to reveal the subcellular localization of circDNAJC11 in MCF-7 cells. H FISH assay was utilized to locate circDNAJC11 in subcellular fractions. Scale bar, 50 μm. I Relative expression of circDNAJC11 in BC cells and normal breast cells was determined by qRT-PCR. For E, F, and I, β-actin was utilized as a loading control. Data were presented as mean ± SD and representative of three independent experiments in E, F, G, and I. E and F were analyzed by Student’s t-test, and (I) was analyzed with ANOVA. **P < 0.01, ***P < 0.001