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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: LINC00543 promotes colorectal cancer metastasis by driving EMT and inducing the M2 polarization of tumor associated macrophages

Fig. 5

LINC00543/miR-506-3p/FOXQ1 regulates EMT of CRC cells. a Intracellular localization of LINC00543 was identified by nuclear/cytoplasmic fractionation experiments and FISH analysis (scale bar = 25 μm). b Polysome Fractionation analysis evaluating the effect of LINC00543 on FOXQ1 translation. c Actinomycin D analysis of the effect of LINC00543 on FOXQ1 mRNA degradation. d Dual luciferase analysis of the effect of LINC00543 on FOXQ1 DNA transcription. e Cycloheximide-chase analysis of the effect of LINC00543 on FOXQ1 protein degradation. f The expression of FOXQ1 and EMT markers were detected in LINC00543 cells upon treatment with miR-506-3p mimics. g Wound-healing assays and h transwell assays in LINC00543 cells transfected with miR-506-3p mimics and control mimics; magnification, × 200

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Journal of Translational Medicine

ISSN: 1479-5876

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