Fig. 8

TTK induces EMT in EC cells by upregulating miR-21 expression. A Correlation of TTK levels with miR-21 expression in TCGA EC (ENCORI). B The expression of miR-21 in TCGA EC and normal samples (UALCAN). ***P < 0.001, by Wilcoxon tests. C MiR-21 levels in EC cells and EM cells as assessed by qRT-PCR. ***P < 0.001, by Student’s t-tests. D The expression of miR-21 in EC cells transfected with TTK expression vector (or control vector), or with TTK shRNA (control shRNA). ***P < 0.001, by Student’s t-tests. E MiR-21 expression was evaluated using qRT-PCR in EC cells transfected with miR-21 mimic (or miR-21 inhibitor). ***P < 0.001, by Student’s t-tests. F, G MTT assays were conducted to examine the effects of miR-21 overexpression (F) or knockdown (G) on TX resistance. ***P < 0.001, by Student’s t-tests. H, I qRT-PCR was performed to measure the expression of ZO-1, VIM, and FN in EC cells transfected with miR-21 mimic (H) or with miR-21 inhibitor (I). ***P < 0.001, by Student’s t-tests