Fig. 4
The levels of DNA/RNA methylation-related genes, as well as the normalized NT5DC3 (5mC CpG island 1/m6A 2309 site). A The levels of DNMT under different concentrations of glucose. B The levels of DNMT with NT5DC3 siRNA treatment. C The levels of NT5DC3 (5mC CpG island 1) under different concentrations of glucose. D The levels of NT5DC3 (5mC CpG island 1) with NT5DC3 siRNA treatment. E The levels of eight m6A related genes (METTL3, METTL14, WTAP, FTO, ALKBH5, YTHDF1, YTHDF2 and YTHDF3) under different concentrations of glucose. F The levels of eight m6A- related genes with NT5DC3 siRNA treatment. This part also verified the results in (E). G The levels of NT5DC3 (m6A 2309) under different concentrations of glucose. H The levels of NT5DC3 (m6A 2309) with NT5DC3 siRNA treatment. I The levels of NT5DC3 (m6A 2309) with the treatment of eight genes siRNA fragments, respectively. J The protein levels of NT5DC3 and HKDC1 with the treatment of eight genes siRNA fragments, respectively. N stands for normal-glucose (2 g·L−1), H stands for high-glucose (5 g·L−1), LF stands for lactoferrin, H-N stands for the transfer from high-glucose to normal-glucose. The above data are presented as mean ± SD, *P < 0.05 compared with the control, & P < 0.05 compared with LF treatment group (n = 3).