Fig. 3

Targeting hepatocyte SGK1 alleviates IR-driven colorectal cancer liver metastasis. A A schematic representation of the experimental design is depicted. We injected SGK1-siRNA mannose-conjugated complex into the tail vein of mice to knock down hepatocytes SGK1 at first. Intrasplenic injection of luciferase-labeled MC38 colorectal cancer cell line was then performed. After 15 min circulation, mice were subjected to liver IR and a splenectomy at the same time. B Representative image of CRLM (n = 6 samples/group). C The number of metastases on liver surface (n = 6 samples/group). D and E Representative image of bioluminescence and statistics were shown (n = 6 samples/group). F Representative histological staining (H&E) of ischemic liver tissue (n = 4samples/group). Scale bars, 500 μm. G Tumor area (n = 6 samples/group). H Survival (n = 8samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001