Fig. 2

AI662270 increases atherosclerotic lesion size and arrests lipid degradation during atherogenesis. A-F Representative images of aortic root cross sections stained with hematoxylin and eosin (H&E) (A) and analysis (B, C, E), and oil red O (D) and analysis (F) from ApoE^−/− mice intravenously injected with Lv-AI662270 and Lv-null or saline fed with HFD or CD for 16 weeks (Scale bar = 400 μm). G-H Representative oil red O staining of aortas and analysis of ApoE^−/− mice injected with Lv-AI662270 and Lv-null or saline fed with HFD or CD for 16 weeks. I CCK-8 assay was performed to measure the effect of AI662270 on macrophages viability. J Tunel positive assay was performed from ApoE^−/− mice intravenously injected with Lv-AI662270 and Lv-null fed with HFD for 16 weeks (Scale bar = 20 μm), “L” represents lumen. n = 6 mice, each dot denotes an individual animal. **p < 0.01 vs. mice in CD. #p < 0.05 vs. Lv-null treated mice in HFD. Data are expressed as mean ± SEM