Fig. 1

Osteosarcoma arises from osteogenesis imperfecta during development. A Single cell RNA-sequence data (scRNA-seq) analysis for 8-week-old long bones (GSE143753), human normal bone tissue (GSE196678) and OS (GSE162454). Cluster analyzed by using Uniform Manifold Approximation and Projection (UMAP) method and populations were identified by color. B Markers used to define subpopulations. C Separated UMAP depending on tissue origins. D Volcano plot of significant different genes (| log2FC | > 1; P < 0.01) between normal osteocytes and OS oncocytes. E, F Enrichment of MGI Mammalian Phenotype and Jensen DISEASES by using the upregulated genes in D (p.value<0.01)