Fig. 3

MiR-26b-5p directly targets PDE4B and CDK8. A–B qRT-PCR was conducted to analyze the expression of PDE4B and CDK8 in cells transfected with miR-26b-5p mimics or inhibitor C–D for 48 h. E–F PDE4B or CDK8 expression was detected in xenografts of nude mice injected with miR-26b-5p agomir or miR-26b-5p antagomir G–H using qRT-PCR. I Western blot analysis of PDE4B and CDK8 protein levels in cells transfected with miR-26b-5p mimics, inhibitor, or controls for 48 h. J–K Luciferase reporter was carried out in HEK-293 T cells co-transfected with dual-luciferase reporter genes containing the wild type (wt) or mutant (mut) CDK8/PDE4B 3’UTR and miR-26b-5p mimics or control mimics. L Schematic representation of putative wild or mutant miR-26b-5p binding sites in PDE4B/CDK8 3’ UTR. Quantitative data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001