Fig. 3

BV enhances VEGFA secretion and angiogenesis via activating PI3K/AKT/mTOR pathway. A The expression of PI3K/AKT/mTOR pathway members were detected by western blot in HCT116 and SW480 treated with or without BV. B Western blot analysis of PI3K/AKT/mTOR pathway members with or without the treatment of BV, LY294002 and Rapamycin in HCT116. C The concentration of VEGFA in the culture medium of control and BV treated HCT116 and SW480 cells with or without the absence of LY294002 and Rapamycin. D HUVEC migration and tube formation in representative images in the CM of HCT116 cocultured with BV with or without the absence of LY294002 and Rapamycin. Scale bar, 50 μm and 100 μm respectively. The migrated HUVEC numbers and increasing folds of tube formation are shown in the bar graph. *, P < 0.05; **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001