Fig. 4

LONP1 K145 is the deacetylation site for Sirt3. A Mass spectrum was conducted to detect the acetylation sites of LONP1. B K145 of LONP1 is conserved among many species. The sequence around LONP1 K145 of several species were aligned. C LONP1-K145Q promotes SW480 cells growth. SW480 cells were stably transfected with vector, LONP1-WT, LONP1-K145Q and LONP1-K145R lentivirus. Cell viability of per group was detected by CCK-8 after 48 h. D, E LONP1-K145Q promotes the migration of SW480 cells. Cells were seeded in transwell. Migrated cells were counted. F–I LONP1-K145Q enhanced mitochondrial OXPHOS function. Mitochondrial function was detected by Cell Mito Stress Test kit using Seahorse analysis F, G. LONP1-K145Q enhanced the level of maximal OCR H and ATP production I of SW480 cells