Fig. 2
From: Sirt3 restricts tumor initiation via promoting LONP1 deacetylation and K63 ubiquitination
LONP1 is deacetylated by Sirt3. A The tumor tissue and paracancerous tissue of patient with colorectal cancer were lysated and the levels of LONP1 and Sirt3 were detected by WB. B, The expression of LONP1 was negatively correlated with Sirt3. R2 = 0.2691, p = 0.0191. C The acetylation of LONP1 was detected in SW480, Hela and U87 cells by WB. AcK, pan-acetyl-lysine antibody. D, WB showing higher level of acetylation of LONP1 after treatmen of 7.5 mM NAM and 5 mM TSA for 24 h in SW480 cells. E The level of acetylation of LONP1 was enhanced after stably knock-down of Sirt3 in SW480 cells. F Elevated LONP1 expression and its acetylation level were detected in AVS compared to APCMin/+ mice. G, H LONP1 interacts with Sirt3 in vivo