Fig. 2

Hypoxia condition induced lactate secretion of cancer cells, further facilitated M2 polarization of macrophages. A Lactate contents in the supernatant of SW480 and HT29 cells were measured by ELISA kit. B mRNA levels and C protein levels of lactate-related genes were detected by RT-qPCR. D Colony formation, E Transwell cell migration and F wound healing assays were performed to assess cell mobility. G M1 and M2 phenotypes of macrophages were examined by flow cytometry assay. CD86 and MHCII were indicators for M1, CD163 and CD206 were indicators for M2. Data were presented as mean ± SD and each assay was performed for three times. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001