Fig. 4

The correlation between tumor-infiltrating T lymphocytes and TAMs of fresh LSCC tissue samples using flow-cytometric analysis (n = 15). A The correlation between CD68⁺ total TAM infiltration and total CD3⁺ T cell A1, CD4⁺ T cell in total CD3⁺ A2, CD8⁺ T cell in total CD3⁺ A3, and CD4⁺/CD8⁺ rate A4; (B) The correlation between CD68⁺CD206⁺ M2 TAM infiltration and total CD3⁺ T cell B1, CD4⁺ T cell in total CD3⁺ B2, CD8⁺ T cell in total CD3⁺ B3, and CD4⁺/CD8⁺ rate B4; C The correlation between CD68⁺HLA-DR^highCD206⁺ M2 subgroup infiltration and total CD3⁺ T cell C1, CD4⁺ T cell in total CD3⁺ C2, CD8⁺ T cell in total CD3⁺ C3, and CD4⁺/CD8⁺ rate C4; D The correlation between CD68⁺HLA-DR^low/−CD206⁺ M2 subgroup infiltration and total CD3⁺ T cell D1, CD4⁺ T cell in total CD3⁺ D2, CD8⁺ T cell in total CD3⁺ D3, and CD4⁺/CD8⁺ rate D4. The presence of costimulatory molecule for T-cell activation including CD40 (first column), CD80 (second column), CD86 (third column) and CD25 (last column) in CD68⁺HLA-DR^low/−CD206⁺ M2, CD68⁺HLA-DR^highCD206⁺ M2, and CD68⁺iNOS⁺ M1 subgroups E, and the scatter plot (with mean ± SD) comparing fractions of the above-mentioned surface antigens within the three TAM subsets F. TAMs, Tumor-associated macrophages. Linear regression analyses were used to test the correlation between different subgroups of cells