Fig. 1

CD206⁺ M2-like macrophage was the dominnat TAM population in the tumor microenvironment of LSCC. A Immunofluorescence staining of CD68⁺iNOS⁺ M1-like TAMs on LSCC tumor sections. iNOS (green), CD68 (red) and DAPI (blue). The corresponding HE staining was shown in the lower right-hand panel. B and C Double-immunofluorescence staining of CD68⁺CD206⁺ and CD68⁺CD163⁺ M2-like TAMs. B CD206 (green) and CD68 (red) staining of LSCC sections. Total nuclei were co-stained with DAPI (blue); The corresponding HE staining was shown in the lower right hand panel. C Two representative double-immunofluorescence staining of CD68⁺CD206⁺ and CD68⁺CD163⁺ TAMs on LSCC tissues. Upper panels showed CD68⁺(red) and CD206⁺ (green) and lower panels showed CD68⁺ (green) and CD163⁺ (red). D The infiltrating level of CD68⁺CD206⁺ TAMs was significantly higher than that of CD68⁺iNOS⁺ in LSCC tissue, p-value < 0.0001; E Representative dot plots displayed CD206⁺ M2 and iNOS⁺ M1 in fresh tumor tissues of LSCC patients using flow-cytometric analysis. The corresponding statistics analysis was shown in F using Paired sample t test, with statistical significance indicated as follows: ****p < 0.0001; G Multiplex immunofluorescence staining of CD68, CD206 and iNOS of LSCC tissue