Fig. 5

Glycoproteomics reveals glycoproteins that may be associated with Fut2-mediated CRC tumorigenesis A Schematic illustration of our systems biology approach to identify N-glycoproteins mediating the effects of Fut2 on CRC metastasis. B The number glycoproteins with fold change over 2.0 in WT vs. Fut2^△IEC mice by proteomic analysis. C The differentially expressed glycoproteins by GO analysis in WT vs. Fut2^△IEC mice. D The differentially expressed glycoproteins by COG analysis in WT vs. Fut2^△IEC mice. E The differentially N-glycosylation expressed glycoproteins presented by the volcano plot in WT vs. Fut2^△IEC mice. F The relative protein level of MCAM in colon tissues from WT vs. Fut2^△IEC mice by proteomic analysis. G The relative N-glycosylation expression level of MCAM in colon tissues from WT vs. Fut2^△IEC mice by glycoproteomic analysis. Data are expressed as mean ± SEM. The data come from three independent experiments. In all panels: ***p < 0.001, ****p < 0.0001. (CRC colorectal cancer, WT wild type, MCAM melanoma cell adhesion molecule, SEM Standard Error of Mean)