Fig. 4

Tumor secretome derived from non-malignant NSCLC cells. Left: Enrichment analysis of the top 10 terms related to biological processes of each cluster according to EnrichR analysis. Right: Heatmap of gene abundance from cell lines (Reads Per Kilobase Million—RPKM) and lung adenocarcinoma derived-organoid duplicates (Fragment Per Kilobase Million—FPKM) in five clusters generated using k-means clustering analysis of secreted and non-secreted encoding genes. Global expression profiles of eight CCLE cell lines (Cancer Cell Line Encyclopedia) derived from adenocarcinoma (N = 4) and squamous cell carcinomas (N = 4) were evaluated. Global gene expression profiles of 3D organoids were collected from a single patient available in the HCMI catalog (Human Cancer Model Initiative)