Fig. 6
Effects of Mreg derived L-EV (L-EVMreg) on in-vitro wound healing and angiogenesis. A. Photomicrographs of a representative wound healing assay employing human endothelial cells (HUVEC) with addition of 106 L-EVMreg/ml and without addition of L-EVMreg. Note the presence of numerous L-EV in the treatment group (arrows). Scale bars denote 250 µm. B. Statistical analysis of the effect of L-EVMreg addition on relative wound closure (control = 1) after 8 h. The column denotes the mean ± SD of 3 independent experiments; *, P < 0.05 (one sample student t-test vs. 1). C. Representative images of tube formation analysis employing HUVEC cell cultures with and without the addition of L-EVMreg. Scale bars denote 500 µm. D. The influence of L-EVMreg addition on various tube formation parameters. Horizontal columns denote the mean ± SD of 3 independent experiments. *, P < 0.05; **, P < 0.01 (one sample student t-test vs. 0). Green color, branches related parameters; yellow color, segments related parameters; cyan color, meshes related parameters; blue color, nodes related parameters; red color, junctions related parameters. For parameter definitions and details on the angiogenesis analyzer tool please refer to: http://image.bio.methods.free.fr/ImageJ/?Angiogenesis-Analyzer-for-ImageJ&lang=en