Fig. 5

Cholesterol compromises nTreg cellular and mitochondrial function. In vitro expanded nTregs were treated with cholesterol alone or in combination with MT for 16 h. A nTregs were co-cultured with CTV labeled Tconv cells for 4 days. MFI of CTV was measured by flow cytometry. n = 4 per group. B, E MMP of nTregs was determined by TMRM staining. C, D mtROS were measured by mitoSOX staining. n = 4 per group. F Oxidative damage of nTregs was evaluated by 8-OHdG ELISA analysis. n = 3 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01