Fig. 3

HIF-1a expression is induced by mtROS. Naïve T cells were polarized for 2 days with cholesterol added alone or combined with MT. A-B mtROS were analyzed with mitoSOX staining. n = 3–4 per group. C Protein levels of HIF-1a were analyzed by western blotting. n = 4 per group. D Relative mRNA levels of HIF-1a, VEGF and GLUT-1 were measured by RT-qPCR. n = 4 per group. E The percentage of Foxp3.⁺ cells at day 2 was measured by flow cytometry. n = 3–4 per group. F HREs-GFP reporter Jurkat cell line was constructed. G HREs-GFP reporter Jurkat cells were stimulated with the indicated reagents for 2 days, and MFI of GFP was measured by flow cytometry. n = 5 per group. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01