Fig. 4

Inhibition of miR-34a-5p alleviated apoptosis of cardiomyocytes induced by ATO. A Inhibition of miR-34a-5p increased the viability of ATO-treated mouse cardiomyocytes. B Inhibition of miR-34a-5p decreased the inhibition rate of ATO-treated mouse cardiomyocytes. C Representative TUNEL staining images of mouse cardiomyocytes. Magnification: 200 × ; scale bar: 100 μm. D Downregulation of miR-34a-5p decreased Bax protein expression in ATO-treated mouse cardiomyocytes. E Downregulation of miR-34a-5p increased Bcl-2 protein expression in ATO-treated mouse cardiomyocytes. F Downregulation of miR-34a-5p increased Sirt1 mRNA expression in ATO-treated mouse cardiomyocytes. G Downregulation of miR-34a-5p increased Sirt1 protein expression in ATO-treated mouse cardiomyocytes. For A, B, D, E, F and G, one-way ANOVA F value = 66.59, 66.59, 17.14, 6.733, 48.78 and 7.761, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 vs. AMO-NC group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. ATO + AMO-NC group; A, B, n = 12; C–G, n = 3–6