Fig. 2

ITGA5 is highly expressed in CD44⁺EpCAM⁺ cells. A The proportion of CD44⁺EpCAM⁺ cells by SP sorting using flow cytometric analysis. B Sphere-forming ability of CD44⁻EpCAM⁻ cells and CD44⁺EpCAM⁺ cells examined by sphere-forming assay (scale bar: 100 μm). C The expression of CD133, CD44, OCT-4, MDR1, EpCAM, ABCG2, and CD24 in CD44⁻EpCAM⁻ cells and CD44⁺EpCAM⁺ cells examined by RT-qPCR. D Expression of ITGA5 in CD44⁻EpCAM⁻ cells and CD44⁺EpCAM⁺ cells examined by RT-qPCR. *p < 0.05 compared with CD44-EpCAM⁻ cells. Measurement data are expressed as mean ± standard deviation. Comparisons between two groups were analyzed using unpaired t-test. The cell experiment was run in triplicate independently