Fig. 1

Bioinformatics prediction of gastric cancer-related miRNAs and genes. A A UpSet plot of the merged GSE2685, GSE13911, GSE19826 and GSE26942 gene expression datasets. The left histogram shows the total amount of elements contained in each original gene expression dataset, the below intersection dot refers to the corresponding datasets on the left, and the connection between the dots indicates the presence of intersection between the corresponding datasets. B UMAP diagram before batch effect removal. C UMAP diagram after batch effect removal. D Venn diagram of the DEGs in gastric cancer tissue samples from GSE2685, GSE13911, GSE19826 and GSE26942 gene expression datasets and the known genes related to gastric cancer from the MalaCards database. E Detection of ITGA5 expression in gastric cancer and adjacent normal tissue by Western blot (N means adjacent normal tissue, T means gastric cancer tissue, sample number is 4). F The expression of miR-148a, miR-148b, and miR-152 in gastric cancer cell lines (MKN45, AGS, KATO-III, NCI-N87, and SNU-1) and normal immortalized gastric mucosal cells GES-1 examined by RT-qPCR. *p < 0.05 compared with adjacent normal tissue samples or GES-1 cells