Fig. 4

The reversed expression of DNB-neighboring genes is driven by DNB genes. A The series of graphs illustrates the pattern of dynamic changes in DNB neighboring genes between Pre-deterioration stage and Deteriorated stage using MFUZZ. B Cytoscape visualization of the interaction network between the DNB genes and their neighboring genes, including three stages of epithelial cell deterioration. The DNB genes are represented by square-shaped network nodes from the pink region. The DNB-neighboring genes are represented by the network nodes grouped in circles from the orange region. All three types of deterioration states involve the same genes and their locations in the network, and a gradient from blue to red denotes low to high levels of gene expression. C Cytoscape visualization of DNB genes and their neighboring genes interaction network in the pre-deterioration stage. Rectangles represent the DNB genes, and circles represent the DNB-neighboring genes. The gradient from blue to red denotes low to high levels of gene expression. D–F The expression of FOS, JUN and TP53 in before-deterioration stage and deteriorated stage. Differences in expression are checked using the Wilcox test. G FOS and JUN regulated the expression of P53 and its downstream apoptosis-related genes to suppress P53-induced apoptosis. The oval represents DNB genes, the rectangle represents DNB-neighboring genes, and the diamond represents additional genes that do not belong to any category. The gradient from blue to red denotes low to high levels of gene expression