Table 1 Forward and reverse primers utilized for qPCR
Gene | Forward primers (5’–3’) | Reverse primers (5’–3’) |
|---|---|---|
Sirt1 | CGGCTACCGAGGTCCATATAC | CCGCAAGGCGAGCATAGATA |
Sirt2 | GAGCCGGACCGATTCAGAC | TTCGAGGGTCAGCTCGTCTA |
Sirt3 | GATTCGGATGGCGCTTGAC | TCTCCCACCTGTAACACTCCC |
Sirt4 | TTTATCCCGGCAAAACCCGA | CCGCTCATTCTTATTCTGTCTGG |
Sirt5 | TCCCCACCGCTTTTTGCAG | GCCATATTTGAACTTGGACGAGC |
Sirt6 | GAGTGTGGGGCTGTCAGT | GGGGCCTCTGAAGTCGGG |
Sirt7 | AGGGTCCAGCTTGAAGGTACTA | CTCGACAGCCTCTTCTGTCTC |
β–actin | CACTGTCGAGTCGCGTCC | TCATCCATGGCGAACTGGTG |