Fig. 1
Tan IIA attenuates SIRT1-mediated ER stress in bEnd0.3 cells. a Chemical structure of Tan IIA. b The mRNA expressions of SIRT1-7. c bEnd0.3 cells were divided into four groups: control, Aβ (Aβ1 − 42 10 µM), Aβ + Tan IIA (Aβ1 − 42 10 µM + Tan IIA 20 µM), Aβ + Tan IIA + EX527 (Aβ1 − 42 10 µM + Tan IIA 20 µM + EX527 10 µM). Western blot analysis (d, e) and indirect immunofluorescence (f) of the expression of SIRT1. g-p Western blot analysis of ER stress: (h) BIP; (i) P-PERK/ PERK; (j) P-eIF-2/ eIF-2α; (k) P-IRE-1α/ IRE-1α; (l) XBP1; (m) PDI; (n) ATF6; and (o) CHOP. p, q Indirect immunofluorescence of P-PERK (p) and P-IRE-1α (q). Scale bar is 50 μm. The mRNA expression was first normalized by the housekeeping gene, and then normalized to the controls. The mean ± SD was calculated based on three separate studies, *P < 0.05, **P < 0.01, NS: no significance