Fig. 7

Overexpression of PAK2 reverses the inhibitory effect of miR-140-3p on gastric cancer. A Western blot analysis of PAK2 in AGS and HGC27 cells subjected to different treatments (miR-NC, miR-140-3p or miR-140-3p + pcDNA-PAK2). B Cell Counting Kit-8 assay was used to detect cell proliferation in AGS and HGC27 cells in different groups (miR-NC, miR-140-3p or miR-140-3p + pcDNA-PAK2). C and D Cell migration and invasion assays in AGS and HGC27 cells in different groups (miR-NC, miR-140-3p or miR-140-3p + pcDNA-PAK2). E Tube formation assay in human umbilical vein endothelial cells incubated with exosome samples from AGS and HGC27 cells subjected to different treatments (miR-NC, miR-140-3p or miR-140-3p + pcDNA-PAK2). F Protein levels of E-cadherin, N-cadherin, vimentin and VEGF-A in AGS and HGC27 cells subjected to different treatments (miR-NC, miR-140-3p or miR-140-3p + pcDNA-PAK2). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. PAK2, p21 activated kinase 2; miR, microRNA; NC, negative control