Fig. 7

cNDC80 enhanced GBM cell proliferation, migration, and invasion, and GSC maintenance through the miR-139-5p/ECE1 pathway. A By using qRT–PCR assays, to determine the expression levels of ECE1 in GBM cells after silencing miR-139-5p alone or in conjunction with the silencing of cNDC80. B ECE1 was analyzed using a Western blot in the specified cells, with GPAPDH acting as a control. C–E GBM cells transfected with sh-cNDC80, anti-miR-139-5p, or anti-miR-139-5p in addition to sh-cNDC80 were examined for proliferation employing the CCK-8 test, the clone formation assay, and the EdU assay. F GBM cells were transfected with sh-cNDC80, anti-miR-139-5p, or anti-miR-139-5p and sh-cNDC80 to study the migration and invasion of the cells by Transwell. G U87 and pGBM-1 cells transfected with sh-cNDC80, anti-miR-139-5p, or anti-miR-139-5p and sh-cNDC80 were examined utilizing the Wound healing test for migration and invasion. H The stemness of GSCs after transduction was assessed by analyzing the percentage of positive wells in a clonogenic assay and by analyzing typical photomicrographs of the new clonal sphere. Each experiment was conducted three times, and the findings are shown as mean ± SD. (**P < 0.01, ***P < 0.001, ****P < 0.0001)