Fig. 6 

ECE1 is a direct target of miR-139-5p. A Using a Venn diagram, it can be shown that miR-139-5p may have 32 mRNA as biological targets. B The expression of miR-139-5p in the glioma patient databank housed in CGGA database. C Using the CGGA database, survival rates were compared between two groups of patients defined by their miR-139-5p levels using the Kaplan–Meier method. D Analyzing ECE1 expression in the CGGA database. E Using the CGGA database, we divided patients with regards to their ECE1 levels and created a Kaplan–Meier survival curve to compare the survival rates between the two groups. F The putative binding sites of miR-139-5p on ECE1. G ECE1 3′UTR reporter constructions, both wild-type and mutant. H, I In GBM cells, the functional relationship between miR-139-5p and ECE1 was confirmed using a luciferase reporter experiment. Each experiment was conducted three times, and the findings are shown as mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)