Fig. 3
From: CircNDC80 promotes glioblastoma multiforme tumorigenesis via the miR-139-5p/ECE1 pathway
cNDC80 enhanced GSC maintenance in vitro. A Following transfection in GBM–GSC cells, Sox2, Oct4, and Nanog were analyzed using Western blots. B CellTiter-Glo assay was utilized to evaluate the cellular impacts in GBM–GSC cells. C The impact of cNDC80 knockdown on the growth of GBM–GSC cells was determined by direct cell counting. D After transfection, GSC stemness was assessed using a clonogenic test. Typical photomicrographs of the newly formed clonal sphere (left) and statistical analysis of the percentage of positive wells (right). Scale bar, 100 µm. Each experiment was conducted three times, and the findings are shown as mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)