Fig. 7

Combination of BRAF inhibitor with MEK inhibitors, RTK inhibitors, or chemotherapy drugs in PTC organoid lines. a Combination drug test of vemurafenib with selumetinib, trametinib, lenvatinib, vandetanib, doxorubicin, and vincristine in PTC-1 organoid line. Each data point represents mean ± SEM of 3 independent biological replicates. Organoid viability was detected by CellTiter-Glo assay after 5 days of drug treatment, and results were calculated relative to DMSO-treated control organoids. b The Combination Index (CI) versus Fractional Effect plot for the indicated drug combinations, according to the Chou-Talalay dose–effect method. The CI < 1.0, CI = 1.0, and CI > 1.0 indicate synergism, additivity, and antagonism, respectively. c Response of each PTC organoid line to vemurafenib, trametinib, or combination therapy. AUC generated from biological replicates of a dose–response curve to drug alone and in combination. d Representative bright-field images of vemurafenib and trametinib-treated PTC organoids (PTC-1_O), alone or in combination. Scale bar, 100 µm