Fig. 6

A STAT3 inhibitor reversed the ability of KPNA2 to promote angiogenesis in vitro. AAdenovirus-infected HUVEC overexpressed KPNA2 and were treated with a STAT3 inhibitor, and tube formation assays were carried out under hypoxia. Images were taken after 6 h. Scale bar: 200 μm. B Total tube length from tube formation assays. C Adenovirus-infected HUVEC overexpressed KPNA2 and were treated with a STAT3 inhibitor before Transwell assays performed under hypoxia. Images were taken after 6 h. Scale bar: 200 μm. D Statistical analysis of the results of Transwell assays (cells/fields). E Adenovirus-infected HUVEC overexpressing KPNA2 were treated with a STAT3 inhibitor and subjected to EdU/DAPI staining under hypoxia (red: EdU, blue: DAPI). Scale bar: 200 μm. F Statistical analysis of the EdU/DAPI staining results. G The binding of STAT3 and KPNA2 promotes the binding of STAT3 and JAK1 and phosphorylation of STAT3. P-STAT3 enters the nucleus, where it promotes the expression of VEGF and ANGPT2, which promotes angiogenesis. Each experiment was repeated three times. * p < 0.05