Fig. 5
ALA upregulated Parkin/LC3B-driven mitophagy in lung fibroblasts. A Transmission electron microscopy analysis showing autophagosome/autolysosome (arrows) after treatment of lung fibroblasts. Bar: 500 μm. B Immunofluorescence analysis of the co-localization of Mitotracker and Lysotracker. Original magnification: 20 μm. C Mitophagy as determined by mt-Keima transfection. Green indicates mitochondria located in the cytoplasm, and red indicates mitochondria located in lysosomes. Original magnification: 50 pixels. D Mitophagy ratios are calculated as the ratio of red area to green area. E mtROS levels as examined using the mitoSOX indicator. Original magnification: 100 μm. F, G Co-localization of LC3B and COX IV or Parkin was assessed by confocal microscopy. Original magnification: 50 pixels and 20 μm. H, I Parkin and LC3B in cell lysate and mitochondrial components. J Co-localization of LC3B, Parkin, and collagen I in lung tissues. Original magnification: 20 μm. Data are presented as mean ± SEM. *P < 0.05 versus control group; #P < 0.05 versus TGF-β1 group (n = 3)