Fig. 5

DoE analysis of T cell isolation parameters and the validation through CAR-T manufacture. This figure shows the results of DoE analysis for the three above parameters and the validation of the optimized T cell selection process with a new round of CAR-T manufacture. a DoE distribution analysis of input factors and output responses. The diagonal pattern shows the desired responses and their distribution in research factors. CD25 (b) and CD69 (c) expression percentage in CD3⁺ cells before CD3⁺ T cell selection and after 48-h T cell activation, live cell diameter changes (d), expansion fold of CAR-T cells after retroviral transduction (e), and CAR expression percentage in CD3⁺ cells at day 3, 6, 9 after retroviral transduction of CAR-T manufacturing process for DoE validation (f)