Fig. 3

Downregulation of SMIM3 inhibited cell proliferation and colony formation in AML cells. The efficiency of SMIM3 knockdown in the Kasumi-1 and THP-1 cell lines was verified by A, B reverse transcription-quantitative polymerase chain reaction and E, F immune fluorescence, respectively. C, D Cell proliferation was detected by the CCK-8 assay in Kasumi-1 and THP-1. G, H The colony formation of SMIM3-CTRL and SMIM3-KD in Kasumi-1 and THP-1. CTRL, control; KD, knockdown; *P < 0.05 compared with CTRL cells; **P < 0.01 compared with CTRL cells; ***P < 0.001 compared with CTRL cells; Error bars indicate the standard deviation